Detection ofMycobacterium aviumsubsp.paratuberculosisby a Direct In Situ PCR Method
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چکیده
منابع مشابه
Detection of Mycobacterium avium subsp. paratuberculosis by a Direct In Situ PCR Method
In situ detection of Mycobacterium avium subsp. paratuberculosis is useful for diagnosis and research of paratuberculosis. The aim of this paper was to detect this agent in formalin-fixed, paraffin-embedded tissue samples by a direct in situ PCR. The technique was performed on ileum or ileocaecal lymph node samples from 8 naturally infected cattle and 1 healthy calf, by using p89 and p92 primer...
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OBJECTIVE To analyze the sequence difference between human A, B, and O alleles and establish the method of ABO genotyping by PCR direct sequencing. METHODS PCR-direct sequencing technique was used to analyze two regions of cDNA from A transferase gene, 233-433 and 660-788. RESULTS Two nucleotide substitutions at 258th and 297th were found in 233-433 region, and a nucleotide substitution at ...
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ژورنال
عنوان ژورنال: Veterinary Medicine International
سال: 2011
ISSN: 2042-0048
DOI: 10.4061/2011/267102